Production and Purification of Peroxidase from Tissue Cultures of Cauliflower (Brassica oleracea L. var. Botrytis)

The Plant peroxidases are remarkable enzymes due to their widespread use in industry. These enzymes, which capable of catalyzing the oxidation various organic and inorganic substrates, have been used clinical diagnosis, detoxification reactions synthesis. In this study, vitro production purification peroxidase enzyme from cauliflower plant was proposed. Firstly, sterile seedlings were obtained MS/B5 nutrient medium without growth regulator seeds calluses containing 0.5 mg / L 2.4-D. Callus powdered with liquid nitrogen then homogenized. Peroxidase purified these homogenates using affinity technique. SDS-PAGE electrophoresis performed determine molecular weight single bands observed at approximately 46 kDa. addition, KM Vmax values callus determined for guaiacol, pyrogallol H2O2 substrates.